Journal: Planta

Article Title: Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-bound β-glucuronosyltransferases from radish primary roots

doi: 10.1007/s00425-013-1959-0

Figure Lengend Snippet: Analysis of the [ 14 C]GlcA transfer product and its enzymatically degraded products by size-exclusion HPLC. Elution profile of the [ 14 C]GlcA transfer product formed from a root AGP digested successively with α- l -arabinofuranosidase and endo-β-(1 → 6)-galactanase as the exogenous acceptor is shown. Dotted lines Intact transfer product, open circles digestion with β-glucuronidase, closed circles digestion with exo-β-(1 → 3)-galactanase and β-glucuronidase. The eluates were fractionated and their radioactivity was counted. The column system was calibrated with high- M r dextran ( V o ) and pullulans with known M r , (Shodex Standard P-82; Showa Denko, Tokyo, Japan), and GlcA

Article Snippet: The column system was calibrated with high- M r dextran ( V o ) and pullulans with known M r , (Shodex Standard P-82; Showa Denko, Tokyo, Japan), and GlcA

Techniques: Radioactivity